Thursday, 15 April 2004
Organisers P.J. Coffer (Utrecht, The Netherlands)
D. Roos (Amsterdam, The Netherlands)
Sponsors GlaxoSmithKline (The Netherlands)
ZonMw (The Netherlands)
KNAW (The Netherlands)

Phagocyte biology: from gene to function

Session I Migration, adhesion and integrin function Thursday Morning
Chairs M. Ginsburg (La Jolla, USA)
I. Maridonneau-Parini (Toulouse, France)
abstract number
08.30 – 08.45 The phagocyte-specific Src tyrosine kinase, Hck, requires the synergic action of its lysosome- and plasma membrane-associated isoforms to induce migratory and tumorigenic abilities of NIH3T3 cells
V. Le Cabec, R. Poincloux, C. Cougoule, and I. Maridonneau-Parini (Toulouse, France)
08.45 – 09.00 C-termini of Rho-like GTPases control differential signaling toward migration
J.P. ten Klooster, P.B. van Hennik, C. Voermans, E.C. Anthony, J.R. Halstead, N. Divecha, and P.L. Hordijk (Amsterdam, The Netherlands)
09.00 – 09.15 Crohn’s disease results from a failure to recruit phagocytes
D.J.B. Marks, B. Al-Lazikani, M. Harbord, S. Bloom, and A.W. Segal (London, UK)
09.15 – 09.30 Characterisation of focal sites in adherent eosinophils
L. Ulfman, M. Lokuta, C. van Aalst, L. Koenderman, and A. Huttenlocher (Utrecht, The Netherlands; Wisconsin, MA, USA)
09.30 – 10.00 Proteomics: an emerging tool in phagocyte research
J.M.F.G. Aerts (Amsterdam, Netherlands)
10.00 end of session
10.00 – 11.15 coffee break + plenary lecture
Session II Oxidative killing mechanisms
Chairs A.W. Segal (London, UK)
D. Roos (Amsterdam, Netherlands)
11.30 – 11.45 How neutrophils kill microbes – an essential role for large-conductance calcium activated channels
J. Ahluwalia, A. Tinker, L.H. Clapp, and A.W. Segal (London, UK)
11.45 – 12.00 NADPH oxidase-associated diaphorase activity supported by the C’-terminal of gp91phox is independent of cPLA2
I. Pessach, T.L. Leto, Z. Shmelzer, M.C. Dinauer, and R. Levy (Beer Sheva, Israel; Indianapolis, USA)
12.00 – 12.15 Mannan-Binding Lectin (MBL)-mediated opsonization and phagocytosis of zymosan
N. Brouwer, R. van Zwieten, M. Hart, L.A. Aarden, D. Roos, T.W. Kuijpers, and K.M. Dolman (Amsterdam, The Netherlands)
12.15 – 12.30 Constitutive phosphorylation of p67PHOX in human neutrophils: regulation by a tyrosine kinase-dependent pathway and extracellular signal-regulated kinase 1/2
P.M. Dang, M.A. Gougerot-Pocidalo, and J. El-Benna (Paris, France)
12.30 – 12.45 Identification of 5 new mutations in the CYBB gene leading to different sub-types of X-linked chronic granulomatous disease
M.J. Stasia, E. Maquet, M. Guillot, D. Tissot Dupont, and F. Morel
(Grenoble, France)
12.45 – 13.00 Unusual late presentation of X-linked Chronic Granulomatous Disease in an 80-year-old female with a novel mutation in CYBB
B. Wolach, R. Gavrieli, Y. Scharf, M. de Boer, and D. Roos (Kfar Saba, Israel; Amsterdam, The Netherlands)
13.00 end of session
13.00 – 15.00 lunch / poster viewing / exhibition + plenary lecture
Session III Innate immune receptors Thursday afternoon
Chairs S. Gordon (Oxford, UK)
T. van den Berg (Amsterdam, The Netherlands)
abstract number
15.00 – 15.45 Macrophage receptors and innate immunity
S. Gordon (Oxford, UK)
15.45 – 16.00 Direct interaction between periplakin and Fc?RI (CD64) controls receptor endocytosis and ligand binding
J.M. Beekman, J. Bakema, J.G.J. van de Winkel, and J.H.W. Leusen (Utrecht, The Netherlands)
16.00 – 16.30 coffee break
16.30 – 16.45 Activation of the NADPH oxidase by intracellular Toll-like receptor-4 signalling in response to S. typhimurium infection
R. van Bruggen, D. Zweers, J. Calafat, N. Borregaard, J.T. van Dissel, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands; Copenhagen, Denmark; Leiden, The Netherlands)
16.45 – 17.00 Cytosolic phospholipase A2 is required for Fc?RII induction during differentiation of PLB cells with 1,25(OH)2D3
Z. Hazan-Eithan, Y. Weinstein, and R. Levy (Beer Sheva, Israel)
17.00 – 17.15 Regulation of the Fca-receptor by cytokine-mediated inside-out signalling
H. Honing, M. Bracke, P.J. Coffer, and L. Koenderman (Utrecht, The Netherlands)
17.15 – 17.30 Filamin A stabilizes cell surface expression of Fc?RI
J.M. Beekman, P.V.E. van den Berghe, D.L.C. van den Berg, J.M. Griffith, M.J. Kleijmeer, J.G.J. van de Winkel, and J.H.W. Leusen (Utrecht, The Netherlands)
17.30 – 17.45 Cross-linking of integrins is not sufficient for full activation of neutrophils: anti-integrin antibodies initiate an Fc -receptor III mediated second signal
Z. Jakus, E. Ligeti, C. A. Lowell, and A. Mócsai (Budapest, Hungary; San Francisco, CA, USA)
17.45 – 18.00 In vitro regulation of human granulocyte Fc? receptors expression by peptide hormones
S. Romero, J.A. Bernal, M. Rosety, F. Briceño, P. Ruiz, D. Garcia-Gil, C. Rivera, and F. Gomez (Cadiz, Spain)
18.00 end of session
Session IV Receptor signaling and recycling Friday morning
Chairs I. Touw (Rotterdam, The Netherlands)
P.J. Coffer (Utrecht, The Netherlands)
abstract number
08.30 – 09.15 G-CSF receptor signaling in normal granulopoiesis, neutropenia and acute myeloid leukemia
I. Touw (Rotterdam, The Netherlands)
09.15 – 09.30 The JAK-STAT pathway is involved in SIRP -induced NO production and transendothelial migration
J. Alblas, H. Honing, C. Renardel de Lavalette, S.M.A. van der Pol, H.E. de Vries, M. Brown, C.D. Dijkstra, and T.K. van den Berg (Amsterdam, The Netherlands; Oxford, United Kingdom)
09.30 – 09.45 Expression and modulation of suppressors of cytokine signalling (SOCS) in human neutrophils
C. Ratthé, and D. Girard (Québec, Canada)
09.45 – 10.00 discussion
10.00 – 11.15 coffee break + plenary lecture
11.30 – 11.45 The immunostimulatory peptide WKYMVm-NH2 activates primary and secondary granule secretion as well as superoxide production of mouse neutrophils via multiple signal transduction pathways
R. Boxio, C. Bossenmeyer-Pourié, R. Vanderesse, C. Dournon, and O. Nüsse (Nancy, France; Orsay, France)
11.45 – 12.00 Impaired priming of phosphatidylinositol 3,4,5-trisphosphate accumulation and Rac activation in neutrophils from patients with Myelodysplasia
G.M. Fuhler, K.A. Cadwallader, G.J. Knol, E.R. Chilvers, A.L. Drayer, and E. Vellenga (Groningen, The Netherlands; Cambridge, UK)
12.00 – 12.15 Calpain activity during beta-2 integrin-mediated phagocytosis
S. Dewitt, and M.B.Hallett (Cardiff, UK)
12.15 – 12.30 The same I?Ba mutation in two related individuals leads to completely different clinical syndromes
R. Janssen, A. van Wengen, M. Hoeve, M. van Tol, R. Bredius, T.H. Ottenhoff, C. Weemaes, J.T. van Dissel, and A. Lankester (Leiden, The Netherlands)
12.30 – 12.45 Prolactin trigger VEGF synthesis and heme oxygenase-1 overexpression in human macrophages
L. Malaguarnera, R.M. Imbesi, M.R. Pilastro, A. Scuto, F. Licata, S. Sanfilippo, and A. Messina (Catania, Italy)
12.45 – 13.00 discussion
13.00 end of session
Session V Regulation of differentiation and apoptosis Saturday morning
Chairs M. Horwitz (Seattle, USA)
C. Haslett (Edinburgh, UK)
abstract number
08.30 – 09.15 Mistrafficking of neutrophil elastase in neutropenia
M. Horwitz (Seattle, USA)
09.15 – 10.00 Granulocyte apoptosis and clearance by macrophages
C. Haslett (Edinburgh, UK)
10.00 – 11.15 coffee break + plenary lecture
11.30 – 11.45 Protein kinase B activity regulates lineage choice decisions during granulopoiesis
M. Buitenhuis, K. Birkenkamp, H. van Deutekom, L. Koenderman, and P. Coffer (Utrecht, The Netherlands)
11.45 – 12.00 Proteinase 3 is involved in caspase 3 activation associated with monocyte differentiation in the absence of apoptosis
M. Pederzoli, and V. Witko-Sarsat (Paris, France)
12.00 – 12.15 p38-Mitogen activated protein kinase and protein phosphatase type 2A regulates caspase-3 activity in human neutrophils
M. Alvarado-Kristensson, F. Melander, L. Rönnstrand, C. Wernstedt, and T. Andersson (Lund, Sweden; Malmö, Sweden; Uppsala, Sweden)
12.15 – 12.30 G-CSF delays neutrophil apoptosis through inhibition of Bid truncation and Bid/Bax targeting to the mitochondria and caspase activation
N.A. Maianski, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands)
12.30 – 12.45 Interleukin-15, but not interleukin-21, delays neutrophil apoptosis and up-regulates CD11b/CD18 expression
M. Pelletier, A. Bouchard, and D. Girard (Pointe-Claire, Canada)
12.45 – 13.00 Caspase-1, a link between mevalonate kinase deficiency and inflammation
L. Kuijk, J. Frenkel, I. Schellens, S.M. Mandey, G.T. Rijkers, H.R. Waterham, and W. Kuis (Utrecht, The Netherlands; Amsterdam, The Netherlands)
13.00 end of session
13.00 – 14.00 lunch / poster viewing / exhibition
Session VI Microbial escape mechanisms Saturday afternoon
Chairs A. Zychlinsky (Berlin, Germany)
J. Swanson (Ann Arbor, USA)
abstract number
14.00 – 14.45 Phagocytes in enteric infections
A. Zychlinsky (Berlin, Germany)
14.45 – 15.00 Novel anti-phagocytic molecules of Staphylococcus aureus
S.H.M. Rooijakkers, W.J.B. van Wamel, T. van Steeg, M. Ruyken, J.S. Presanis, H.C.J.M. Heezius, R.B. Sim, K.P.M. van Kessel, and J.A.G. van Strijp (Utrecht, The Netherlands)
15.00 – 15.30 coffee break
15.30 – 16.15 Activated macrophages alter phagosomes to prevent Listeria monocytogenes from escaping into cytoplasm
J. T. Myers, R. M. Henry, L. Shaughnessy, and J. A. Swanson (Ann Arbor, USA)
16.15 – 16.30 Identifying the active sites within the Chemotaxis Inhibitory Protein of Staphylococcus aureus
P.J. Haas, C.J.C. de Haas, K.P.M van Kessel, and J.A.G van Strijp (Utrecht, The Netherlands)
16.30 – 16.45 The Salmonella typhimurium protein SspJ is indispensable for virulence in mice
T. van der Straaten, A. van Diepen, R. Janssen, and J.T. van Dissel (Leiden, The Netherlands)
16.45 – 17.00 final remarks
17.00 end of workshop
ß2-agonists and glucocorticosteroids inhibit neutrophil migration through a bilayer of endothelial and epithelial cells
A.M.Sadowska, F.J. van Overveld, and W.A. De Backer (Antwerp, Belgium)
Binding of exogenous proteinase 3 to plasma membranes of HEK- 293 and promyelocytic leukemia PLB-985 and NB4 cell lines
A. David, U. Specks, and I. Aviram (Tel Aviv, Israel; Rochester, MN, USA)
First report on a child with a novel mutation leading to chronic granulomatous disease and with T-cell acute lymphoblastic leukemia
B. Wolach, S. Ash, R. Gavrieli, B. Stark, I. Yaniv, and D. Roos (Tel Aviv, Israel; Amsterdam, The Netherlands)
Severe clinical forms of cytochrome b-negative chronic granulomatous disease (X91-) in three brothers with a point mutation in the promoter region of CYBB
M.J. Stasia, X.J. Li, J.P. Brion, J. Boutonnat, and F. Morel (Grenoble, France)
Chemotaxis inhibiting protein of Staphylococcus aureus binds to the N-terminus of the C5a receptor
B. Postma, W. Kleibeuker, M. Boonstra, M.J.J.G. Poppelier, K.P.M. van Kessel, J.A.G. van Strijp, and C.J.C. de Haas (Utrecht, The Netherlands)
A superoxide-hypersusceptible Salmonella typhimurium mutant is attenuated but regains virulence in p47phox-/- mice
A. van Diepen, T. van der Straaten, R. Janssen, and J.T. van Dissel (Leiden, The Netherlands)
Identification and characterization of IgA receptor interacting proteins
J.E. Bakema, A. Bakker, S. den Hartog-Jager, S. de Haij, J.G.J van de Winkel, and J.H.W. Leusen (Utrecht, The Netherlands)
Methodological pitfall undermines reliability of studies on neutrophil microbicidal activity
P. Dri, and E. Decleva (Trieste , Italy)
Changes in calcium and pH inside Listeria-containing phagosomes
L. Shaughnessy, A. Hoppe, K. Christensen, and J. Swanson (Ann Arbor, MI, USA)
Phosphorylation of the NADPH oxidase component p67PHOX by ERK2 and p38MAPK
P.M. Dang, F. Morel, M.A. Gougerot-Pocidalo, and J. El Benna (Grenoble, France)
Regulation of macrophage Fc? receptors expression by angiotensin receptor antagonists
S. Romero, J.A. Bernal, P. Ruiz, M. Rosety, F. Briceño, D. Garcia-Gil, C. Rivera, and F. Gomez (Cadiz, Spain)
Macropahge Fc? receptors expression: synergy of statins and angiotensin receptor type II antagonists
S. Romero, J.A. Bernal, P. Ruiz, M. Rosety, D. Garcia-Gil, F. Briceño, C. Rivera, and F. Gomez (Cadiz, Spain)
The structure of CARD15, and molecular mechanisms of mutations associated with granulomatous diseases
D.J.B. Marks, A.W. Segal, and B. Al-Lazikani (London, UK)
Proteomic analysis of neutrophil cytoskeleton
P. Xu, M. Crawford, J. Godovac-Zimmermann, M. Radulovic, and A.W. Segal (London, UK)
Differential impairment of neutrophil function and apoptosis by HIV protease inhibitors
N. Hadad, R. Levy, F. Schlaeffer, and K. Riesenberg (Beer Sheva, Israel)
Gene expression profiles to identify asthma phenotypes for optimising individualised pharmacotherapy
W. ten Hove, H.D. Luijk, L. Koenderman, H.G.M. Leufkens, and M. Bracke (Utrecht, The Netherlands)
Effect of PR-39 on platelet ROS production and platelet-neutrophil aggregation
D. Gritti, R. Malinverni, A. Malinverno, D. Culacciati, C. Ross and G. Ricevuti (Pavia, Italy; Kansas, USA)