Phagocytes

11 – 13 April, 2002

Organizers:
P.L. Hordijk (Amsterdam, The Netherlands)
L. Dekker (London, UK)

Sponsors:
Molecular Probes, The Netherlands
Dutch Asthma Foundation, The Netherlands
Sanquin Research at CLB, The Netherlands
Lark Technologies Inc., United Kingdom
Fisher Scientific, United Kingdom
The Physiological Society, United Kingdom
CGD Research Trust, United Kingdom

Phagocytes: Molecular basis of cell function
Session I Host-Pathogen interactions/Toll like receptors
Thursday, 11 April 2002
Chair M. Thelen (Bellinzona, Switzerland)
Abstract no
9.45 – 10.15 Innate immunity receptors: from IL-1 to pathogen recognition
A. Mantovani (Milan, Italy)
10.15 – 10.45 Myeloid cell receptors and immunity
S. Gordon (Oxford, UK)
10.45 – 11.15 coffee break
11.15 – 11.30 Helicobacter-induced homotypic phagosome fusion in human monocytes is temperature- and wortmannin-sensitive
M.G. Rittig, B. Shaw, D.P. Letley and J.C. Atherton (Nottingham, UK)
95
11.30 – 11.45 A sub-population of Glycopeptidolipids located at the outer capsular surface of Mycobacterium smegmatis specifically inhibits phagocytosis of mycobacteria by human macrophages
C. Villeneuve, V. Abadie, C. Astarie-Dequeker, G. Etienne, C. Bordier, M. Daffé, and I. Maridonneau-Parini (Toulouse, France)
126
11.45 – 12.00 Endocytic pathway during the phagocytosis of conidia of Aspergillus fumigatus by alveolar macrophages
O. Ibrahim- Granet, B. Philippe, H. Boleti, E. Boisvieux-Ullrich, D. Grenet, M. Stern, and J.P. Latgé (Paris, France)
96
12.00 – 12.15 N-Formylpeptide receptor subtypes in human neutrophils activate L-plastin phosphorylation through different signal transduction intermediates
M.-H. Paclet, C. Davis, P. Kotsonis, A.W. Segal and L.V. Dekker (London, UK)
97
12.15 – 12.30 Caspase-independent cell death in human neutrophils induced by tumor necrosis factor a (TNF-a)
N.A. Maianski, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands)
98
12.30 end of session I
12.30 – 14.00 lunch and poster viewing
Session II Phagocyte signal transduction
Thursday, 11 April 2002
Chair L. Dekker (London, UK)

Abstract no
15.00 – 15.30 Chemokine receptor signalling
M. Thelen (Bellinzona, Switzerland)
15.30 – 16.00 Phosphoinositide kinases in allergy and inflammation
M. Wymann (Fribourg, Germany)
71
16.00 – 16.30 Role of small GTP-binding proteins of the Rho and ARF families in membrane and actin dynamics during phagocytosis
P. Chavrier (Paris, France)
16.30 –17.00 Regulation of Phosphoinositide levels by ARFs, phospholipase D and PITPs
S. Cockcroft (London, UK)
17.00 end of session II
Session III Adhesion/transmigration
Friday, 12 April 2002
Chair P. Hordijk (Amsterdam, The Netherlands)

Abstract no
9.30 – 10.10 Leukocyte Transendothelial Migration: A junctional affair
F. W. Luscinskas (Boston, USA)
10.15 – 10.45 Potential regulators of interendothelial cell contacts
D. Vestweber (Muenster, Germany)
10.45 – 11.15 coffee break
11.15 – 11.30 VCAM-1-mediated ROS production modulates transmigration of CD34+- cells
J.D. van Buul, C. Voermans, S. van Wetering, E. Mul, C.E. van der Schoot, and P.L. Hordijk (Amsterdam, The Netherlands)
99
11.30 – 11.45 Analysis of beta2 integrin-mediated adhesion of in-vitro differentiated myeloid cell lines HL60 and NB4
M. Bouaouina, E. Blouin, P. Rieu, and L. Halbwachs-Mecarelli (Paris, France)
100
11.45 – 12.00 Blocking of the Intercellular Adhesion Molecule-1 (ICAM-1). Use of antisense oligonucleotides and ICAM-1 antibodies
A.M. Sadowska, F.J. van Overveld, and W.A. De Backer (Antwerp, Belgium)
101
12.00 – 12.15 Neutrophil respiratory burst and tissue penetration are normal in Crohns disease during G-CSF adminitration
M. Harbord, R. Day, S. Bloom, A. Forbes, and A.W. Segal (London, UK)
12.15 – 12.30 Defective adherent activation, but not transmigration, in Syk-deficient neutrophils
A. Mócsai, V. Tybulewicz, and C. A. Lowell (London, UK)
102
12.30 end of session III
12.30 – 14.00 lunch and poster viewing
Session IV Granules and phagocyte function
Saturday, 13 April 2002
Chair D. Roos, (Amsterdam, The Netherlands)

Abstract no
9.30 – 10.05 Targeting and processing of neutrophil granule proteins
I. Olsson (Lund, Sweden)
10.05 – 10.45 New aspects of novel neutrophil granule proteins
N. Borregaard (Copenhagen, Denmark)
10.45 – 11.15 coffee break
11.15 – 11.30 Neutrophil secretion requires ion influx across the plasma membrane
D. Granfeldt, O. Harbecke, A. Karlsson, and C.Dahlgren (Göteborg, Sweden)
103
11.30 – 11.45 NADPH Oxidase: determination of its FAD binding sites
R. van Bruggen, C. Meischl, E. Anthony, M. de Boer, and D. Roos (Amsterdam, The Netherlands)
104
11.45 – 12.00 Inhibition of NADPH-oxidase by phosphorylated p40phox in an amphiphile-free phosphorylated p47phox dependent activation system. Role of Threonine 154
L. Rossetti-Lopes*, M-C Dagher*, A-P Bouin, A. Fuchs and B.M. Babior (*contributed equally) (S Paulo, Brazil / Grenoble, France / California, USA)
105
12.00 – 12.15 Stimulation of phagocytic cells induces translocation of cytosolic phospholipase A2 to plasma membranes and its association with NADPH oxidase
R. Levy, Z. Smeltzer, E. Admon and M. Hershfinked (Beer Sheva, Israel)
126A
12.15 – 12.30 Different expression pattern of matrix metalloproteinases by Epstein-Barr Virus immortalized B lymphocytes from healthy donors and chronic granulomatous disease patients
C. Trocmé, L. Grange, M.J. Stasia, P. Birembaut, P. Gaudin and F. Morel (Grenoble, France)
106
12.30 end of session IV
12.30 – 14.00 lunch and poster viewing
Session V NADPH oxidase and phagocyte disorders
Saturday, 13 April 2002
Chair D. Lambeth (Atlanta, USA)

Abstract no
14.00 – 14.40 The role of potassium in NADPH oxidase mediated killing
T. Segal (London, UK)
14.40 – 15.15 NADPH oxidase and regulation by the Rho GTPase Rac2: Studies in vitro and in knockout mice
M. Dinauer (Indianapolis, USA)
15.15 – 15.45 coffee break
15.45 – 16.20 Cytoskeletal disturbances in the Wiskott-Aldrich syndrome
A. Thrasher (London, UK)
16.20 – 16.40 A proteomics approach for evaluation of intracellular oxidative killing mechanisms of neutrophils
M. Nagl, E. Reeves, V. Soskic, and A.W. Segal (London, UK
108
16.40 – 17.00 Final remarks
17.00 end of workshop 10
Workshop 10  Posters

Abstract no
Interaction of neutrophil CD11b/CD18 with proteinase 3
A. David , Y. Kacher, and I. Aviram (Tel Aviv, Israel)
109
Sequential migration of neutrophils across monolayers of endothelial and epithelial cells
M. Lorenowicz, F.P.J. Mul, A.E.M. Zuurbier, H. Janssen, J. Calafat, S. van Wetering, P.S. Hiemstra, D. Roos and P.L. Hordijk (Amsterdam / Leiden, The Netherlands)
110
Participation of Rac GTPase activating proteins in the deactivation of the phagocytic NADPH oxidase
P. Moskwa, M.C. Dagher,M.H. Paclet, F. Morel, and E. Ligeti (Budapest, Hungary)
111
Human granulocyte Fc? receptors expression regulation by peptide hormones in vitro
F. Briceño, M. Cousinou, S. Romero, I. Trouillhet, C. Rivera and F. Gomez (Cadiz, Spain)
112
Treatment with angiotensin converting enzyme inhibitors regulate macrophage Fc? receptors expression
F. Briceño, J.A. Bernal, M. Herran, A. Garcia-Egido, M. Escobar, C. Rivera, and F. Gomez
(Cadiz, Spain)
113
Synergistic effects of angiotensin converting enzyme inhibitors and statins regulating macropahge Fc? receptors expression
F. Briceño, F.J. Fernandez, F.M. Orihuela, S. Romero, C. Rivera, and F. Gomez (Cadiz, Spain)
114
Determination of the elastase activity in the supernatant of human neutrophils
A. Vocks, M. Petkovic and J. Arnhold (Leipzig, Germany)
115
Leukosialin (CD43) down-regulation during neutrophil apoptosis may be related to membrane blebbing
P. Nusbaum, P. Lesavre, and L.Halbwachs-Mecarelli (Paris, France)
116
Study of killing of conidia of Aspergillus fumigatus by alveolar macrophages
B. Philippe, O. Ibrahim-Granet, M. Stern, D. Grenet, and J.P. Latgé (Paris, France)
117
Characterisation of the neutrophil secretory phospholipase A2 by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS)
M. Petkovic, J. Müller, M. Müller, J. Schiller, K. Arnold and J. Arnhold (Leipzig, Germany)
118
Specific granules of human eosinophilic leukocytes have lysosomal characteristics
T. Persson, P. Andersson, J. Calafat, H. Janssen, M. Karawajczyk, S.R. Carlsson, and
A. Egesten (Lund / Malmö / Uppsala / Umeå, Sweden / Amsterdam, The Netherlands)
119
Cleavage of cell surface sialic acid, primes the neutrophil for galectin-3 activation
J. Almkvist, C.Dahlgren, H. Leffler, and A. Karlsson (Lund, Sweden)
120
Degranulation and apoptosis are two distinct mechanisms leading to proteinase 3 membrane expression independently of its serine-protease activity in stably transfected mast cell lines
S. Durant, S. Canteloup, P. Nusbaum, M. Pederzoli, L. Halbwachs-Mecarelli,
and V. Witko-Sarsat (Paris, France)
121
G-CSF and GM-CSF preserve functional abilities of aging neutrophils
L.J.W. van der Laan/B. Wolach, K. Maianski, A.T.J. Tool, D. Roos, and T.W. Kuijpers
(Kfar Saba, Israel / Amsterdam, The Netherlands)
122
Effect of AT-III preincubation on PMN aggregation and CD11b/CD18 up-regulation
G. Ricevuti, A. Malinverno and D. Gritti (Pavia, Italy)
123
Does H+ transport by gp91phox occur by a proton-wire mechanism ?
T.J. Mankelow, X.W. Hu, K. Adams and L.M. Henderson (Bristol, UK)
124
Live Helicobacter pylori as well as HP-NAP induce neutrophil transendothelial migration
M. Innocenti, S. Lundin, A. Karlsson, A-M. Svennerholm and M. Quiding-Järbrink
(Göteborg, Sweden)
125