11 – 13 April, 2002

P.L. Hordijk (Amsterdam, The Netherlands)
L. Dekker (London, UK)

Molecular Probes, The Netherlands
Dutch Asthma Foundation, The Netherlands
Sanquin Research at CLB, The Netherlands
Lark Technologies Inc., United Kingdom
Fisher Scientific, United Kingdom
The Physiological Society, United Kingdom
CGD Research Trust, United Kingdom

Phagocytes: Molecular basis of cell function

Session I Host-Pathogen interactions/Toll like receptors
Thursday, 11 April 2002

Chair M. Thelen (Bellinzona, Switzerland)
Abstract no
9.45 – 10.15 Innate immunity receptors: from IL-1 to pathogen recognition
A. Mantovani (Milan, Italy)
10.15 – 10.45 Myeloid cell receptors and immunity
S. Gordon (Oxford, UK)
10.45 – 11.15 coffee break
11.15 – 11.30 Helicobacter-induced homotypic phagosome fusion in human monocytes is temperature- and wortmannin-sensitive
M.G. Rittig, B. Shaw, D.P. Letley and J.C. Atherton (Nottingham, UK)
11.30 – 11.45 A sub-population of Glycopeptidolipids located at the outer capsular surface of Mycobacterium smegmatis specifically inhibits phagocytosis of mycobacteria by human macrophages
C. Villeneuve, V. Abadie, C. Astarie-Dequeker, G. Etienne, C. Bordier, M. Daffé, and I. Maridonneau-Parini (Toulouse, France)
11.45 – 12.00 Endocytic pathway during the phagocytosis of conidia of Aspergillus fumigatus by alveolar macrophages
O. Ibrahim- Granet, B. Philippe, H. Boleti, E. Boisvieux-Ullrich, D. Grenet, M. Stern, and J.P. Latgé (Paris, France)
12.00 – 12.15 N-Formylpeptide receptor subtypes in human neutrophils activate L-plastin phosphorylation through different signal transduction intermediates
M.-H. Paclet, C. Davis, P. Kotsonis, A.W. Segal and L.V. Dekker (London, UK)
12.15 – 12.30 Caspase-independent cell death in human neutrophils induced by tumor necrosis factor a (TNF-a)
N.A. Maianski, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands)
12.30 end of session I
12.30 – 14.00 lunch and poster viewing
Session II Phagocyte signal transduction
Thursday, 11 April 2002
Chair L. Dekker (London, UK)

Abstract no
15.00 – 15.30 Chemokine receptor signalling
M. Thelen (Bellinzona, Switzerland)
15.30 – 16.00 Phosphoinositide kinases in allergy and inflammation
M. Wymann (Fribourg, Germany)
16.00 – 16.30 Role of small GTP-binding proteins of the Rho and ARF families in membrane and actin dynamics during phagocytosis
P. Chavrier (Paris, France)
16.30 –17.00 Regulation of Phosphoinositide levels by ARFs, phospholipase D and PITPs
S. Cockcroft (London, UK)
17.00 end of session II
Session III Adhesion/transmigration
Friday, 12 April 2002
Chair P. Hordijk (Amsterdam, The Netherlands)

Abstract no
9.30 – 10.10 Leukocyte Transendothelial Migration: A junctional affair
F. W. Luscinskas (Boston, USA)
10.15 – 10.45 Potential regulators of interendothelial cell contacts
D. Vestweber (Muenster, Germany)
10.45 – 11.15 coffee break
11.15 – 11.30 VCAM-1-mediated ROS production modulates transmigration of CD34+- cells
J.D. van Buul, C. Voermans, S. van Wetering, E. Mul, C.E. van der Schoot, and P.L. Hordijk (Amsterdam, The Netherlands)
11.30 – 11.45 Analysis of beta2 integrin-mediated adhesion of in-vitro differentiated myeloid cell lines HL60 and NB4
M. Bouaouina, E. Blouin, P. Rieu, and L. Halbwachs-Mecarelli (Paris, France)
11.45 – 12.00 Blocking of the Intercellular Adhesion Molecule-1 (ICAM-1). Use of antisense oligonucleotides and ICAM-1 antibodies
A.M. Sadowska, F.J. van Overveld, and W.A. De Backer (Antwerp, Belgium)
12.00 – 12.15 Neutrophil respiratory burst and tissue penetration are normal in Crohns disease during G-CSF adminitration
M. Harbord, R. Day, S. Bloom, A. Forbes, and A.W. Segal (London, UK)
12.15 – 12.30 Defective adherent activation, but not transmigration, in Syk-deficient neutrophils
A. Mócsai, V. Tybulewicz, and C. A. Lowell (London, UK)
12.30 end of session III
12.30 – 14.00 lunch and poster viewing
Session IV Granules and phagocyte function
Saturday, 13 April 2002
Chair D. Roos, (Amsterdam, The Netherlands)

Abstract no
9.30 – 10.05 Targeting and processing of neutrophil granule proteins
I. Olsson (Lund, Sweden)
10.05 – 10.45 New aspects of novel neutrophil granule proteins
N. Borregaard (Copenhagen, Denmark)
10.45 – 11.15 coffee break
11.15 – 11.30 Neutrophil secretion requires ion influx across the plasma membrane
D. Granfeldt, O. Harbecke, A. Karlsson, and C.Dahlgren (Göteborg, Sweden)
11.30 – 11.45 NADPH Oxidase: determination of its FAD binding sites
R. van Bruggen, C. Meischl, E. Anthony, M. de Boer, and D. Roos (Amsterdam, The Netherlands)
11.45 – 12.00 Inhibition of NADPH-oxidase by phosphorylated p40phox in an amphiphile-free phosphorylated p47phox dependent activation system. Role of Threonine 154
L. Rossetti-Lopes*, M-C Dagher*, A-P Bouin, A. Fuchs and B.M. Babior (*contributed equally) (S Paulo, Brazil / Grenoble, France / California, USA)
12.00 – 12.15 Stimulation of phagocytic cells induces translocation of cytosolic phospholipase A2 to plasma membranes and its association with NADPH oxidase
R. Levy, Z. Smeltzer, E. Admon and M. Hershfinked (Beer Sheva, Israel)
12.15 – 12.30 Different expression pattern of matrix metalloproteinases by Epstein-Barr Virus immortalized B lymphocytes from healthy donors and chronic granulomatous disease patients
C. Trocmé, L. Grange, M.J. Stasia, P. Birembaut, P. Gaudin and F. Morel (Grenoble, France)
12.30 end of session IV
12.30 – 14.00 lunch and poster viewing
Session V NADPH oxidase and phagocyte disorders
Saturday, 13 April 2002
Chair D. Lambeth (Atlanta, USA)

Abstract no
14.00 – 14.40 The role of potassium in NADPH oxidase mediated killing
T. Segal (London, UK)
14.40 – 15.15 NADPH oxidase and regulation by the Rho GTPase Rac2: Studies in vitro and in knockout mice
M. Dinauer (Indianapolis, USA)
15.15 – 15.45 coffee break
15.45 – 16.20 Cytoskeletal disturbances in the Wiskott-Aldrich syndrome
A. Thrasher (London, UK)
16.20 – 16.40 A proteomics approach for evaluation of intracellular oxidative killing mechanisms of neutrophils
M. Nagl, E. Reeves, V. Soskic, and A.W. Segal (London, UK
16.40 – 17.00 Final remarks
17.00 end of workshop 10
Workshop 10  Posters

Abstract no
Interaction of neutrophil CD11b/CD18 with proteinase 3
A. David , Y. Kacher, and I. Aviram (Tel Aviv, Israel)
Sequential migration of neutrophils across monolayers of endothelial and epithelial cells
M. Lorenowicz, F.P.J. Mul, A.E.M. Zuurbier, H. Janssen, J. Calafat, S. van Wetering, P.S. Hiemstra, D. Roos and P.L. Hordijk (Amsterdam / Leiden, The Netherlands)
Participation of Rac GTPase activating proteins in the deactivation of the phagocytic NADPH oxidase
P. Moskwa, M.C. Dagher,M.H. Paclet, F. Morel, and E. Ligeti (Budapest, Hungary)
Human granulocyte Fc? receptors expression regulation by peptide hormones in vitro
F. Briceño, M. Cousinou, S. Romero, I. Trouillhet, C. Rivera and F. Gomez (Cadiz, Spain)
Treatment with angiotensin converting enzyme inhibitors regulate macrophage Fc? receptors expression
F. Briceño, J.A. Bernal, M. Herran, A. Garcia-Egido, M. Escobar, C. Rivera, and F. Gomez
(Cadiz, Spain)
Synergistic effects of angiotensin converting enzyme inhibitors and statins regulating macropahge Fc? receptors expression
F. Briceño, F.J. Fernandez, F.M. Orihuela, S. Romero, C. Rivera, and F. Gomez (Cadiz, Spain)
Determination of the elastase activity in the supernatant of human neutrophils
A. Vocks, M. Petkovic and J. Arnhold (Leipzig, Germany)
Leukosialin (CD43) down-regulation during neutrophil apoptosis may be related to membrane blebbing
P. Nusbaum, P. Lesavre, and L.Halbwachs-Mecarelli (Paris, France)
Study of killing of conidia of Aspergillus fumigatus by alveolar macrophages
B. Philippe, O. Ibrahim-Granet, M. Stern, D. Grenet, and J.P. Latgé (Paris, France)
Characterisation of the neutrophil secretory phospholipase A2 by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS)
M. Petkovic, J. Müller, M. Müller, J. Schiller, K. Arnold and J. Arnhold (Leipzig, Germany)
Specific granules of human eosinophilic leukocytes have lysosomal characteristics
T. Persson, P. Andersson, J. Calafat, H. Janssen, M. Karawajczyk, S.R. Carlsson, and
A. Egesten (Lund / Malmö / Uppsala / Umeå, Sweden / Amsterdam, The Netherlands)
Cleavage of cell surface sialic acid, primes the neutrophil for galectin-3 activation
J. Almkvist, C.Dahlgren, H. Leffler, and A. Karlsson (Lund, Sweden)
Degranulation and apoptosis are two distinct mechanisms leading to proteinase 3 membrane expression independently of its serine-protease activity in stably transfected mast cell lines
S. Durant, S. Canteloup, P. Nusbaum, M. Pederzoli, L. Halbwachs-Mecarelli,
and V. Witko-Sarsat (Paris, France)
G-CSF and GM-CSF preserve functional abilities of aging neutrophils
L.J.W. van der Laan/B. Wolach, K. Maianski, A.T.J. Tool, D. Roos, and T.W. Kuijpers
(Kfar Saba, Israel / Amsterdam, The Netherlands)
Effect of AT-III preincubation on PMN aggregation and CD11b/CD18 up-regulation
G. Ricevuti, A. Malinverno and D. Gritti (Pavia, Italy)
Does H+ transport by gp91phox occur by a proton-wire mechanism ?
T.J. Mankelow, X.W. Hu, K. Adams and L.M. Henderson (Bristol, UK)
Live Helicobacter pylori as well as HP-NAP induce neutrophil transendothelial migration
M. Innocenti, S. Lundin, A. Karlsson, A-M. Svennerholm and M. Quiding-Järbrink
(Göteborg, Sweden)