3 – 5 April 2003
Organizers Prof. G. Berton (Verona, Italy)
Prof. M. Cassatella (Verona, Italy)

Molecular and cellular biology of phagocytes

Session I Innate immunity Thursday
Chairs S.W. Edwards (Liverpool, UK) & A. Albini (Genova, Italy) Abstract No.
09.45 – 10.30 Efferocytosis: phagocytic clearance of apoptotic cells and related mechanisms for host-parasite interaction
P. Henson (Denver, USA)
116
10.30 – 10.45 Phenotypical and functional heterogeneity of human lung macrophages
F. Granata, B. Balestrieri, A. Petraroli, G. Giannatasio, P. Morabito, and M. Triggiani (Naples, Italy)
114
10.45 – 11.00 Expression and function of Toll like receptors in human neutrophils
R. van Bruggen, D. Zweers, A.T.J. Tool, L.W. van der Laan, T.W. Kuijpers, and D. Roos (The Netherlands)
115
11.00 – 11.15 coffee break
11.15 – 12.00 New insights on neutrophils in the angiogenic process
A. Albini, R. Benelli, M. Morini, and D. Noonan (Genova, Italy)
119
12.00 – 12.15 Involvement of IRF-1 in CD95 mediated apoptosis in monocytic cells
E. Conte, L. Manzella, and A. Messina (Catania, Italy)
117
12.15 – 12.30 Role of the cyclin-dependent kinase inhibitor P21/waf1 in neutrophil apoptosis: regulation by its cleavage by proteinase 3
S. Canteloup, V. Gausson, S. Durant, and V. Witko-Sarsat (Paris, France)
118
12.30 – 12.45 Normal G-protein-coupled receptor signaling in Syk-deficient neutrophils and mast cells
A. Mócsai, H. Zhang, Z. Jakus, J. Kitaura, T. Kawakami, and C.A. Lowell (Budapest, Hungary/ San Francisco, CA, USA/San Diego, CA, USA)
158
12.45 – 13.00 Modulation of human dendritic cell responses by sphingosine 1-phosphate
C. Pizzirani, M. Idzko, E. Panther, S. Corinti, A. Morelli, D. Ferrari, F. Di Virgilio, G. Girolomoni, and J. Norgauer (Ferrara, Italy/Freiburg, Germany/Rome, Italy)
148
13.00 End of Session I
Lunch / poster viewing
Session II Phagocyte-cytokine networks Thursday
Chairs F. Bazzoni (Verona, Italy) & D. Roos (Amsterdam, The Netherlands) Abstract No.
15.00 – 15.45 Influencing innate and adaptive immunity by manipulating macrophage cytokine production
D. Mosser (College Park, USA)
15.45 – 16.00 Mechanisms involved in interleukin-15 activation of human neutrophils
M. Pelletier, C. Ratthé, and D. Girard (Québec, Canada)
124
16.00 – 16.15 Regulation of IL-12 p40 and p70 production by blood and alveolar phagocytes during severe sepsis
F. Ethuin, C. Delarche, M.A. Gougerot-Pocidalo, L. Jacob, and S. Chollet-Martin (Paris, France)
122
16.00-16.15 coffee break
16.15 – 17.00 Role of the transcription factor STAT3 and of its isoforms alpha and beta in mediating cytokine signalling in macrophages as well as in other cell types
V. Poli (Turin, Italy)
17.00 – 17.15 Inhibitory effects of interleukin 10 on macrophage activation triggered by toll receptor agonists: role of suppressor of cytokine signaling (SOCS)-3
C. Berlato, L. Gatto, M. Cassatella, and F. Bazzoni (Verona, Italy)
123
17.15 – 17.30 Cytokine regulation in myocardial ischemic disease
E. Vecile, A. Abbate, R. Cattaruzzi, G. DiSciascio, and A. Dobrina (Rome, Italy)
125
17.30 – 17.45 Characterization of the mechanism of action of the CXCR1 inhibitor repertaxin
E. Galliera, R. Bertini, M. Allegretti, M. Locati, A. Mantovani, and F. Colotta (Milan, Italy)
126
18.00 End of Session II
Session III Signal transduction Friday
Chairs T. Andersson (Malmö, Sweden) & A. Gougerot-Pocidalo (Paris, France) Abstract No.
9.40 – 10.20 The diverse roles of phospholipase A2 isotypes in phagocytes
R. Levy (Beer-Sheva, Israel)
10.20 – 10.35 Regulation of neutrophil chemotaxis by cyclic GMP-dependent protein kinase 1 (cGKI)
C. Werner, V. Godfrey, F. Hofmann, and K.B Pryzwansky (München, Germany/Chapel Hill, USA)
127
10.35 – 10.50 Survival signalling by p38-mitogen-activated protein kinase can occur through phosphorylation of caspases
M. Alvarado-Kristensson, F. Melander, and T. Andersson (Malmö, Sweden)
128
10.50 – 11.00 Regulation of apoptosis by Forkhead (FOXO) transcription factors
P.J. Coffer, K.U. Birkenkamp, P.F. Dijkers, E.W.-F. Lam, and L. Koenderman (Utrecht, The Netherlands)
158C
11.00 – 11.15 coffee break
11.15 – 12.00 A retro look at tyrosine kinases and neutrophil activation
P. Naccache (Lavalle, Canada)
12.00 – 12.15 SRC-dependent signaling is a key step in the process of autoregulation of MAC-1 adhesive function in human PMN
P. Piccardoni, L. Totani, S. Manarini, Z. Bagoly, R. Pecce, A. Piccoli, C. Cerletti, and V. Evangelista (Santa Maria Imbaro, Italy)
129
12.15 – 12.30 Avidity regulation of integrin CR3 (CD11b/CD18) in macrophages
J. Chinegwundoh, H. Janssen, J. Calafat, and S. Greenberg (New York, USA/Amsterdam, The Netherlands)
130
12.30 – 12.45 Down-regulation of Rac activity during ß2 integrin-mediated adhesion of human neutrophils
K. Dib, F. Melander, L. Axelsson, M.-C. Dagher, P. Aspenström, and T. Andersson (Malmö, Sweden/Grenoble, France/Uppsala, Sweden)
131
12.45 – 13.00 Modulation of ß2-integrin affinity and avidity
V. Patcha, J. Wigren, M. Winberg, and E. Särndahl (Linköping, Sweden)
132
13.00 End of Session III
Session IV Adhesion, phagocytosis and cytoskeleton dynamics Saturday
Chairs V. Witko-Sarsat (Paris, France) & O. Stendahl (Linköping, Sweden) Abstract No.
9.40 – 10.30 Mechanisms of inside-out control of adhesion of leukocytes to endothelial cells
L. Koenderman (Utrecht, The Netherlands)
10.30 – 10.45 Regulation of macrophage movement by Fgr, Hck and Cbl
E. Caveggion, S. Continolo, F. Pixley, R.E. Stanley, D.D.L. Bowtell, C.A. Lowell, and G. Berton (Verona, Italy/New York, USA/Melbourne, Australia/San Francisco, USA)
133
10.45 – 11.00 Inhibition of phagosomal maturation by lipophosphoglycan (LPG) from Leishmania donovani is dependent on lipid rafts in human macrophages
Å. Holm, M.Lerm, K.-E. Magnusson, A. Descoteaux, and B. Rasmusson (Linköping, Sweden/Laval, QC, Canada)
134
11.00-11.15 coffee break
11.15 – 12.00 p59Hck and p61Hck play distinct roles in human phagocytes
I. Maridonneau-Parini (Toulouse, France)
12.00 – 12.15 Chlamydia Pneumoniae binds to resting human platelets and triggers P-selectin expression and aggregation
M. Majeed, H. Johansson, and T. Bengtsson (Linköping, Sweden)
158B
12.15 – 12.30 Detection of Mannose Binding Lectin (MBL) mediated phagocytosis
K.M. Dolman, M.H. de Blauw, K.E. van Baal, A.T.J. Tool, R. van Zwieten, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands)
136
12.30 – 12.45 Bacterial response to conditions in the neutrophil phagosome
H. Rosen, B.J. Staudinger, M.A. Oberdoerster, P.J. Lewis, and J.R. Johnson (Minnesota, USA)
137
12.45 End of Session IV
Session V NADPH oxidase Saturday
Chairs M. Markert (Lausanne, Switzerland) & S. Dusi (Verona, Italy) Abstract No.
14.15 – 15.00 p67phox-Rac1 chimeras require both liberty and fraternity to activate the NADPH oxidase
E. Pick (Tel Aviv, Israel)
138
15.00 – 15.15 New PLB X+CGD cellular models for the study of mutations’ impact on NADPH oxidase activity
M.J. Stasia, C. Bionda, X. J. Li, R. van Bruggen, B. Polack, D. Roos, and F. Morel (CHU Grenoble, France/Amsterdam, The Netherlands)
139
15.15 – 15.30 The stimulation of human monocytes with interferon-gamma induces the phosphorylation of PU.1 and its binding to gp91phox promoter via activation of a classical protein kinase C
S. Dusi, P. Mazzi, D. Margotto, F. Wientjes*, and M. Donini (Verona, Italy/London, UK )
140
15.30 – 15.45 Structural and functional analysis of the cytoplasmic domains in gp91phox and Nox1
T.K. Epperson, A.J. Valente, K.-H. Krause, and R.A. Clark (San Antonio, TX, USA)
158D
16.00 – 16.15 coffee break
16.15 – 17.00 Activation of the phagocyte NADPH oxidase by phosphatidic acid
L. McPhail (Winston-Salem, USA)
17.00- 17.15 MRP8 and MRP14 activate NADPH oxidase via a direct interaction with cytochrome b558
M. H. Paclet, S. Berthier, A. W. Coleman, F. Roux, S. Vergnaud, and F. Morel (Grenoble, France/Lyon, France)
141
17.15 – 17.30 Tosyl Phenylalanine Chloromethyl Ketone inhibits NADPH oxidase activation by preventing p47phox phosphorylation and translocation
M. Gillibert, Z. Derhy, M. Terrier, J. El Benna, and F. Lederer (Gif-sur-Yvette, France/Paris, France)
142
17.30 – 17.45 Absence of leukocyte glucose-6-phosphate dehydrogenase (G6PD) does not affect the neutrophil bactericidal activity
B. Wolach, M. Ashkenazi, R. Grossmann, Z. Friedman, R. Gavrieli, and D. Roos (Kfar Saba, Tel-Aviv, Israel/Amsterdam, The Netherlands)
144
17.45 – 18.00 Zinc-induced intra-cellular translocation of fatty-acid binding protein p34, S100A8 and S100A9 to the membrane of human neutrophils
M. Markert, R. Hotz, M. Vaglio, J. Mauël, and G. Siegenthaler (Lausanne, Switzerland/Epalinges, Switzerland)
145
18.00 End of workshop

Posters

121
G-CSF-stimulated neutrophils are a prominent source of functional BLYS
P. Scapini, B. Nardelli, G. Nadali, F. Calzetti, G. Pizzolo, and M.A. Cassatella (Verona, Italy/Rockville, MD, USA)

135
Surfactant protein A modulates Mycobacterium tuberculosis uptake by cholesterol-depleted human leukocytes
M. Morosini, F. Meloni, A. Marone Bianco, E. Paschetto, A. Malinverno, and A. Fietta (Pavia, Italy)

143
Oligomannan suppresses neutrophil and monocyte respiratory burst: a possible mechanism for granulomatous inflammation in Crohn’s Disease
C.M. Mpofu, J.M. Rhodes, B.J. Campbell, and S.W. Edwards (Liverpool, UK)

146
Role of Hck and Fgr in the neutrophil response to FMLP
L. Fumagalli, C.A. Lowell, and G. Berton (Verona, Italy/San Francisco, USA)

147
Ethanol-induced inhibition of cytokine release and protein degranulation in human neutrophils
J. Taïeb, C. Delarche, F. Ethuin, S. Selloum, T. Poynard, MA. Gougerot-Pocidalo, and S. Chollet-Martin (Paris, France)

149
Chemokine expression by lymphatic endothelial celIs
E. Vecile, S. Mancardi, N. Dusetti, G. Stanta, O. Burrone, and A. Dobrina (Trieste, Italy)

150
Lipophosphoglycan (LPG) from Leishmania donovani inhibits phagolysosome fusion in human neutrophils
M. Lindmark, C. Andersson, Å. Holm, O. Stendahl, and B. Rasmusson (Linköping, Sweden)

151
Generation of biologically active angiostatin kringle 1-3 by activated human neutrophils
P. Scapini, and M.A. Cassatella (Verona, Italy)

152
Different recognition and scavenging of native and truncated CCL22 by the chemokine receptor D6
R. Bonecchi, M. Locati, M. Vulcano, M. Sironi, A. Fra, M. Gobbi, A. Vecchi, S. Sozzani, and A. Mantovani (Milan, Italy)

154
Transient decrease in neutrophil migration after aerobic exercise
R. Gavrieli, S. Geffen Ben-Dror, A. Eliakim, B. Falk, Y. Radnai, and B. Wolach (Kfar Saba, Israel/Tel-Aviv, Israel)

155
Production of oxygen radicals in phatogen-activated dendritic cells
E. Riboldi, M. Vulcano, D. Lissandrini , P. Mazzi , S. Dusi , and S. Sozzani (Verona, Italy/Brescia, Italy)

156
Regulation of the chemokine receptor CXCR4 by hypoxia
T. Schioppa, A. Saccani, S. Bernasconi, A. Mantovani, and A. Sica (Milan, Italy)

157
Apoptotic neutrophils in the circulation of patients with glycogen storage disease type 1b (GSD1b)
T.W. Kuijpers, N.A. Maianski, A.T.J. Tool, G.P.A. Smit, J.P. Rake, D. Roos, and G. Visser (Amsterdam, The Netherlands/Groningen, The Netherlands/Utrecht, The Netherlands)