3 – 5 April 2003
Organizers Prof. G. Berton (Verona, Italy)
Prof. M. Cassatella (Verona, Italy)

Molecular and cellular biology of phagocytes

Session I Innate immunity Thursday
Chairs S.W. Edwards (Liverpool, UK) & A. Albini (Genova, Italy) Abstract No.
09.45 – 10.30 Efferocytosis: phagocytic clearance of apoptotic cells and related mechanisms for host-parasite interaction
P. Henson (Denver, USA)
10.30 – 10.45 Phenotypical and functional heterogeneity of human lung macrophages
F. Granata, B. Balestrieri, A. Petraroli, G. Giannatasio, P. Morabito, and M. Triggiani (Naples, Italy)
10.45 – 11.00 Expression and function of Toll like receptors in human neutrophils
R. van Bruggen, D. Zweers, A.T.J. Tool, L.W. van der Laan, T.W. Kuijpers, and D. Roos (The Netherlands)
11.00 – 11.15 coffee break
11.15 – 12.00 New insights on neutrophils in the angiogenic process
A. Albini, R. Benelli, M. Morini, and D. Noonan (Genova, Italy)
12.00 – 12.15 Involvement of IRF-1 in CD95 mediated apoptosis in monocytic cells
E. Conte, L. Manzella, and A. Messina (Catania, Italy)
12.15 – 12.30 Role of the cyclin-dependent kinase inhibitor P21/waf1 in neutrophil apoptosis: regulation by its cleavage by proteinase 3
S. Canteloup, V. Gausson, S. Durant, and V. Witko-Sarsat (Paris, France)
12.30 – 12.45 Normal G-protein-coupled receptor signaling in Syk-deficient neutrophils and mast cells
A. Mócsai, H. Zhang, Z. Jakus, J. Kitaura, T. Kawakami, and C.A. Lowell (Budapest, Hungary/ San Francisco, CA, USA/San Diego, CA, USA)
12.45 – 13.00 Modulation of human dendritic cell responses by sphingosine 1-phosphate
C. Pizzirani, M. Idzko, E. Panther, S. Corinti, A. Morelli, D. Ferrari, F. Di Virgilio, G. Girolomoni, and J. Norgauer (Ferrara, Italy/Freiburg, Germany/Rome, Italy)
13.00 End of Session I
Lunch / poster viewing
Session II Phagocyte-cytokine networks Thursday
Chairs F. Bazzoni (Verona, Italy) & D. Roos (Amsterdam, The Netherlands) Abstract No.
15.00 – 15.45 Influencing innate and adaptive immunity by manipulating macrophage cytokine production
D. Mosser (College Park, USA)
15.45 – 16.00 Mechanisms involved in interleukin-15 activation of human neutrophils
M. Pelletier, C. Ratthé, and D. Girard (Québec, Canada)
16.00 – 16.15 Regulation of IL-12 p40 and p70 production by blood and alveolar phagocytes during severe sepsis
F. Ethuin, C. Delarche, M.A. Gougerot-Pocidalo, L. Jacob, and S. Chollet-Martin (Paris, France)
16.00-16.15 coffee break
16.15 – 17.00 Role of the transcription factor STAT3 and of its isoforms alpha and beta in mediating cytokine signalling in macrophages as well as in other cell types
V. Poli (Turin, Italy)
17.00 – 17.15 Inhibitory effects of interleukin 10 on macrophage activation triggered by toll receptor agonists: role of suppressor of cytokine signaling (SOCS)-3
C. Berlato, L. Gatto, M. Cassatella, and F. Bazzoni (Verona, Italy)
17.15 – 17.30 Cytokine regulation in myocardial ischemic disease
E. Vecile, A. Abbate, R. Cattaruzzi, G. DiSciascio, and A. Dobrina (Rome, Italy)
17.30 – 17.45 Characterization of the mechanism of action of the CXCR1 inhibitor repertaxin
E. Galliera, R. Bertini, M. Allegretti, M. Locati, A. Mantovani, and F. Colotta (Milan, Italy)
18.00 End of Session II
Session III Signal transduction Friday
Chairs T. Andersson (Malmö, Sweden) & A. Gougerot-Pocidalo (Paris, France) Abstract No.
9.40 – 10.20 The diverse roles of phospholipase A2 isotypes in phagocytes
R. Levy (Beer-Sheva, Israel)
10.20 – 10.35 Regulation of neutrophil chemotaxis by cyclic GMP-dependent protein kinase 1 (cGKI)
C. Werner, V. Godfrey, F. Hofmann, and K.B Pryzwansky (München, Germany/Chapel Hill, USA)
10.35 – 10.50 Survival signalling by p38-mitogen-activated protein kinase can occur through phosphorylation of caspases
M. Alvarado-Kristensson, F. Melander, and T. Andersson (Malmö, Sweden)
10.50 – 11.00 Regulation of apoptosis by Forkhead (FOXO) transcription factors
P.J. Coffer, K.U. Birkenkamp, P.F. Dijkers, E.W.-F. Lam, and L. Koenderman (Utrecht, The Netherlands)
11.00 – 11.15 coffee break
11.15 – 12.00 A retro look at tyrosine kinases and neutrophil activation
P. Naccache (Lavalle, Canada)
12.00 – 12.15 SRC-dependent signaling is a key step in the process of autoregulation of MAC-1 adhesive function in human PMN
P. Piccardoni, L. Totani, S. Manarini, Z. Bagoly, R. Pecce, A. Piccoli, C. Cerletti, and V. Evangelista (Santa Maria Imbaro, Italy)
12.15 – 12.30 Avidity regulation of integrin CR3 (CD11b/CD18) in macrophages
J. Chinegwundoh, H. Janssen, J. Calafat, and S. Greenberg (New York, USA/Amsterdam, The Netherlands)
12.30 – 12.45 Down-regulation of Rac activity during ß2 integrin-mediated adhesion of human neutrophils
K. Dib, F. Melander, L. Axelsson, M.-C. Dagher, P. Aspenström, and T. Andersson (Malmö, Sweden/Grenoble, France/Uppsala, Sweden)
12.45 – 13.00 Modulation of ß2-integrin affinity and avidity
V. Patcha, J. Wigren, M. Winberg, and E. Särndahl (Linköping, Sweden)
13.00 End of Session III
Session IV Adhesion, phagocytosis and cytoskeleton dynamics Saturday
Chairs V. Witko-Sarsat (Paris, France) & O. Stendahl (Linköping, Sweden) Abstract No.
9.40 – 10.30 Mechanisms of inside-out control of adhesion of leukocytes to endothelial cells
L. Koenderman (Utrecht, The Netherlands)
10.30 – 10.45 Regulation of macrophage movement by Fgr, Hck and Cbl
E. Caveggion, S. Continolo, F. Pixley, R.E. Stanley, D.D.L. Bowtell, C.A. Lowell, and G. Berton (Verona, Italy/New York, USA/Melbourne, Australia/San Francisco, USA)
10.45 – 11.00 Inhibition of phagosomal maturation by lipophosphoglycan (LPG) from Leishmania donovani is dependent on lipid rafts in human macrophages
Å. Holm, M.Lerm, K.-E. Magnusson, A. Descoteaux, and B. Rasmusson (Linköping, Sweden/Laval, QC, Canada)
11.00-11.15 coffee break
11.15 – 12.00 p59Hck and p61Hck play distinct roles in human phagocytes
I. Maridonneau-Parini (Toulouse, France)
12.00 – 12.15 Chlamydia Pneumoniae binds to resting human platelets and triggers P-selectin expression and aggregation
M. Majeed, H. Johansson, and T. Bengtsson (Linköping, Sweden)
12.15 – 12.30 Detection of Mannose Binding Lectin (MBL) mediated phagocytosis
K.M. Dolman, M.H. de Blauw, K.E. van Baal, A.T.J. Tool, R. van Zwieten, D. Roos, and T.W. Kuijpers (Amsterdam, The Netherlands)
12.30 – 12.45 Bacterial response to conditions in the neutrophil phagosome
H. Rosen, B.J. Staudinger, M.A. Oberdoerster, P.J. Lewis, and J.R. Johnson (Minnesota, USA)
12.45 End of Session IV
Session V NADPH oxidase Saturday
Chairs M. Markert (Lausanne, Switzerland) & S. Dusi (Verona, Italy) Abstract No.
14.15 – 15.00 p67phox-Rac1 chimeras require both liberty and fraternity to activate the NADPH oxidase
E. Pick (Tel Aviv, Israel)
15.00 – 15.15 New PLB X+CGD cellular models for the study of mutations’ impact on NADPH oxidase activity
M.J. Stasia, C. Bionda, X. J. Li, R. van Bruggen, B. Polack, D. Roos, and F. Morel (CHU Grenoble, France/Amsterdam, The Netherlands)
15.15 – 15.30 The stimulation of human monocytes with interferon-gamma induces the phosphorylation of PU.1 and its binding to gp91phox promoter via activation of a classical protein kinase C
S. Dusi, P. Mazzi, D. Margotto, F. Wientjes*, and M. Donini (Verona, Italy/London, UK )
15.30 – 15.45 Structural and functional analysis of the cytoplasmic domains in gp91phox and Nox1
T.K. Epperson, A.J. Valente, K.-H. Krause, and R.A. Clark (San Antonio, TX, USA)
16.00 – 16.15 coffee break
16.15 – 17.00 Activation of the phagocyte NADPH oxidase by phosphatidic acid
L. McPhail (Winston-Salem, USA)
17.00- 17.15 MRP8 and MRP14 activate NADPH oxidase via a direct interaction with cytochrome b558
M. H. Paclet, S. Berthier, A. W. Coleman, F. Roux, S. Vergnaud, and F. Morel (Grenoble, France/Lyon, France)
17.15 – 17.30 Tosyl Phenylalanine Chloromethyl Ketone inhibits NADPH oxidase activation by preventing p47phox phosphorylation and translocation
M. Gillibert, Z. Derhy, M. Terrier, J. El Benna, and F. Lederer (Gif-sur-Yvette, France/Paris, France)
17.30 – 17.45 Absence of leukocyte glucose-6-phosphate dehydrogenase (G6PD) does not affect the neutrophil bactericidal activity
B. Wolach, M. Ashkenazi, R. Grossmann, Z. Friedman, R. Gavrieli, and D. Roos (Kfar Saba, Tel-Aviv, Israel/Amsterdam, The Netherlands)
17.45 – 18.00 Zinc-induced intra-cellular translocation of fatty-acid binding protein p34, S100A8 and S100A9 to the membrane of human neutrophils
M. Markert, R. Hotz, M. Vaglio, J. Mauël, and G. Siegenthaler (Lausanne, Switzerland/Epalinges, Switzerland)
18.00 End of workshop


G-CSF-stimulated neutrophils are a prominent source of functional BLYS
P. Scapini, B. Nardelli, G. Nadali, F. Calzetti, G. Pizzolo, and M.A. Cassatella (Verona, Italy/Rockville, MD, USA)

Surfactant protein A modulates Mycobacterium tuberculosis uptake by cholesterol-depleted human leukocytes
M. Morosini, F. Meloni, A. Marone Bianco, E. Paschetto, A. Malinverno, and A. Fietta (Pavia, Italy)

Oligomannan suppresses neutrophil and monocyte respiratory burst: a possible mechanism for granulomatous inflammation in Crohn’s Disease
C.M. Mpofu, J.M. Rhodes, B.J. Campbell, and S.W. Edwards (Liverpool, UK)

Role of Hck and Fgr in the neutrophil response to FMLP
L. Fumagalli, C.A. Lowell, and G. Berton (Verona, Italy/San Francisco, USA)

Ethanol-induced inhibition of cytokine release and protein degranulation in human neutrophils
J. Taïeb, C. Delarche, F. Ethuin, S. Selloum, T. Poynard, MA. Gougerot-Pocidalo, and S. Chollet-Martin (Paris, France)

Chemokine expression by lymphatic endothelial celIs
E. Vecile, S. Mancardi, N. Dusetti, G. Stanta, O. Burrone, and A. Dobrina (Trieste, Italy)

Lipophosphoglycan (LPG) from Leishmania donovani inhibits phagolysosome fusion in human neutrophils
M. Lindmark, C. Andersson, Å. Holm, O. Stendahl, and B. Rasmusson (Linköping, Sweden)

Generation of biologically active angiostatin kringle 1-3 by activated human neutrophils
P. Scapini, and M.A. Cassatella (Verona, Italy)

Different recognition and scavenging of native and truncated CCL22 by the chemokine receptor D6
R. Bonecchi, M. Locati, M. Vulcano, M. Sironi, A. Fra, M. Gobbi, A. Vecchi, S. Sozzani, and A. Mantovani (Milan, Italy)

Transient decrease in neutrophil migration after aerobic exercise
R. Gavrieli, S. Geffen Ben-Dror, A. Eliakim, B. Falk, Y. Radnai, and B. Wolach (Kfar Saba, Israel/Tel-Aviv, Israel)

Production of oxygen radicals in phatogen-activated dendritic cells
E. Riboldi, M. Vulcano, D. Lissandrini , P. Mazzi , S. Dusi , and S. Sozzani (Verona, Italy/Brescia, Italy)

Regulation of the chemokine receptor CXCR4 by hypoxia
T. Schioppa, A. Saccani, S. Bernasconi, A. Mantovani, and A. Sica (Milan, Italy)

Apoptotic neutrophils in the circulation of patients with glycogen storage disease type 1b (GSD1b)
T.W. Kuijpers, N.A. Maianski, A.T.J. Tool, G.P.A. Smit, J.P. Rake, D. Roos, and G. Visser (Amsterdam, The Netherlands/Groningen, The Netherlands/Utrecht, The Netherlands)